Detection by PCR of a VNTR polymorphism at D4S43
نویسندگان
چکیده
منابع مشابه
PCR detection of a biallelic polymorphism at D4S43.
Source and Description: The DNA marker pKP 1.65, is a single copy probe for the D4S43 locus. The 1.7 kb PstI fragment reveals a VNTR (1). Recently, the detection of the VNTR by PCR, after sequencing the adjacent 1269 bp PstI fragment was described (2). In order to construct primers for PCR we have sequenced the 1652 bp PstI fragment of pKP 1.65. A guanosine rich sequence in the last third of th...
متن کاملextremal region detection guided by maxima of gradient magnitude
a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
PCR detection of the TAQ1 polymorphism at the CA2 locus.
In humans, at least seven isozymes of carbonic anhydrase (CA1—CA7) are known, five of which are encoded by genes on chromosomes 1 (CA6), 8 (CA1-3) and 16 (CAT) (1). We have previously described a Taql RFLP with a 50% allele frequency near the CA2 gene (2). We have now bracketed the site with PCR (3) primers to develop an STS site. The original DNA samples (2) produced the same typings (+ = Taql...
متن کاملMolecular characterization of Sardinian Mycobacterium tuberculosis isolates by IS6110 restriction fragment length polymorphism, MIRU-VNTR and rep-PCR.
An evaluation of the utility of rep PCR typing compared to the 15 loci discriminatory set of MIRU-VNTR was undertaken. Twenty-nine isolates of Mycobacterium tuberculosis from patients were examined. Genomic DNA was extracted from the isolates by standard method. The number of copies of tandem repeats of the 15 MIRU-VNTR loci was determined by PCR amplification and agarose gel electrophoresis of...
متن کاملPCR detection of a TaqI polymorphism at the CCAATT box of the human tyrosinase (TYR) gene.
Altered response to growth rate changes in Kluyveromyces lactis versus Saccharomyces cerevisiae as demonstrated by heterologous expression of ribosomal protein 59 {CRY1) Figures 5 and 6 of this article were inadvertantly transposed. The legend to figure 5 should accompany figure 6 as labelled and the legend to figure 6 should accompany figure 5.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.17.4772